CPT® Code 87626

Infectious agent detection by nucleic acid (DNA or RNA); Human Papillomavirus (HPV), separately reported high-risk types (eg, 16, 18, 31, 45, 51, 52) and high-risk pooled result(s)

The CPT® Code 87626 pertains to the detection of infectious agents through nucleic acid testing, specifically targeting the Human Papillomavirus (HPV). HPV is a virus that can invade the skin and mucosal epithelia, leading to both clinical and subclinical infections. There are over 100 known types of HPV, with approximately 40 types predominantly affecting the anogenital tract. Among these, certain high-risk oncogenic types, such as HPV types 16, 18, 31, 45, 51, and 52, are often associated with high-grade squamous intraepithelial lesions (HSIL) and invasive cancers. Conversely, low-risk HPV types are linked to conditions such as acuminate condylomas and low-grade squamous intraepithelial lesions (LSIL) of the cervix. The detection of HPV through nucleic acid testing has become a standard practice in managing cervical disease in women. The testing process involves collecting exfoliated cervical cells using tools like a cytobrush, swab, or plastic spatula, which are then suspended in a liquid transport medium. The sample undergoes treatment with sodium hydroxide to denature the DNA, followed by hybridization with two mixtures of non-isotope single-stranded RNA probes—one for low-risk HPV types and another for high-risk types. The resulting hybridized products are transferred to a microplate with antibody-coated wells that specifically recognize the HPV DNA/RNA hybrids. An alkaline phosphatase-labeled monoclonal antibody is added, along with a chemiluminescent substrate, and the emitted light is measured using a luminometer to determine the ratio of reactivity. This code is specifically utilized when testing for singular high-risk HPV types along with pooled results, ensuring accurate identification and management of potential HPV-related diseases.