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Official Description

Cholesterol, serum or whole blood, total

© Copyright 2026 American Medical Association. All rights reserved.

Common Language Description

A laboratory test known as CPT® Code 82465 is utilized to measure the total cholesterol levels present in serum or whole blood. Cholesterol is a waxy, fat-like steroid substance that is an essential component of cell membranes across all body tissues and organs. The body primarily synthesizes cholesterol, with a smaller portion obtained through dietary sources. This lipid plays a crucial role in the formation of hormones that are vital for growth, development, and reproductive functions. Additionally, cholesterol is involved in the production of bile acids, which are necessary for the digestion of food and the absorption of nutrients. Elevated levels of cholesterol in the bloodstream can indicate an increased risk for cardiovascular diseases and strokes. Furthermore, certain individuals may possess a genetic predisposition that leads to elevated cholesterol levels. The test is performed by obtaining a blood sample, which can be collected through a separately reportable venipuncture or a fingerstick method. The analysis of serum, plasma, and whole blood is conducted using a quantitative enzymatic method to ensure accurate measurement of cholesterol levels.

© Copyright 2026 Coding Ahead. All rights reserved.

1. Indications

The cholesterol test, identified by CPT® Code 82465, is performed for several specific indications, which include the following:

  • Risk Assessment for Cardiovascular Disease Elevated cholesterol levels may indicate an increased risk for cardiovascular diseases, prompting the need for monitoring and management.
  • Stroke Risk Evaluation High cholesterol is also associated with a higher risk of stroke, making this test important for individuals with risk factors.
  • Genetic Predisposition Individuals with a family history of high cholesterol or related conditions may require testing to assess their cholesterol levels.

2. Procedure

The procedure for obtaining a cholesterol measurement using CPT® Code 82465 involves several key steps, which are detailed below:

  • Step 1: Patient Preparation Prior to the test, patients may be advised to fast for a specific period, typically 9 to 12 hours, to ensure accurate results. This fasting period helps to eliminate the influence of recent food intake on cholesterol levels.
  • Step 2: Sample Collection A blood sample is collected from the patient. This can be done through a venipuncture, where a needle is inserted into a vein, or via a fingerstick, which involves pricking the finger to obtain a small blood sample. The method of collection may depend on the clinical setting and the patient's condition.
  • Step 3: Laboratory Analysis The collected serum, plasma, or whole blood sample is then sent to a laboratory for analysis. The laboratory employs a quantitative enzymatic method to measure the total cholesterol levels accurately. This method involves using specific reagents that react with cholesterol to produce measurable results.

3. Post-Procedure

After the cholesterol test is completed, patients may be advised to resume their normal activities immediately. There are typically no specific post-procedure care instructions required, as the test is minimally invasive. However, patients should be informed about the importance of discussing their results with their healthcare provider to understand their cholesterol levels and any necessary follow-up actions or lifestyle modifications that may be recommended based on the findings.

Short Descr ASSAY BLD/SERUM CHOLESTEROL
Medium Descr CHOLESTEROL SERUM/WHOLE BLOOD TOTAL
Long Descr Cholesterol, serum or whole blood, total
Status Code Statutory Exclusion (from MPFS, may be paid under other methodologies)
Global Days XXX - Global Concept Does Not Apply
PC/TC Indicator (26, TC) 9 - Not Applicable
Multiple Procedures (51) 9 - Concept does not apply.
Bilateral Surgery (50) 9 - Concept does not apply.
Physician Supervisions 09 - Concept does not apply.
Assistant Surgeon (80, 82) 9 - Concept does not apply.
Co-Surgeons (62) 9 - Concept does not apply.
Team Surgery (66) 9 - Concept does not apply.
Diagnostic Imaging Family 99 - Concept Does Not Apply
CLIA Waived (QW) Yes
APC Status Indicator Service Paid under Fee Schedule or Payment System other than OPPS
Type of Service (TOS) 5 - Diagnostic Laboratory
Berenson-Eggers TOS (BETOS) T1H - Lab tests - other (non-Medicare fee schedule)
MUE 1
CCS Clinical Classification 233 - Laboratory - Chemistry and Hematology
59 Distinct procedural service: under certain circumstances, it may be necessary to indicate that a procedure or service was distinct or independent from other non-e/m services performed on the same day. modifier 59 is used to identify procedures/services, other than e/m services, that are not normally reported together, but are appropriate under the circumstances. documentation must support a different session, different procedure or surgery, different site or organ system, separate incision/excision, separate lesion, or separate injury (or area of injury in extensive injuries) not ordinarily encountered or performed on the same day by the same individual. however, when another already established modifier is appropriate it should be used rather than modifier 59. only if no more descriptive modifier is available, and the use of modifier 59 best explains the circumstances, should modifier 59 be used. note: modifier 59 should not be appended to an e/m service. to report a separate and distinct e/m service with a non-e/m service performed on the same date, see modifier 25.
QW Clia waived test
90 Reference (outside) laboratory: when laboratory procedures are performed by a party other than the treating or reporting physician or other qualified health care professional, the procedure may be identified by adding modifier 90 to the usual procedure number.
GA Waiver of liability statement issued as required by payer policy, individual case
GZ Item or service expected to be denied as not reasonable and necessary
GW Service not related to the hospice patient's terminal condition
GY Item or service statutorily excluded, does not meet the definition of any medicare benefit or, for non-medicare insurers, is not a contract benefit
QJ Services/items provided to a prisoner or patient in state or local custody, however the state or local government, as applicable, meets the requirements in 42 cfr 411.4 (b)
Q5 Service furnished under a reciprocal billing arrangement by a substitute physician or by a substitute physical therapist furnishing outpatient physical therapy services in a health professional shortage area, a medically underserved area, or a rural area
Q1 Routine clinical service provided in a clinical research study that is in an approved clinical research study
91 Repeat clinical diagnostic laboratory test: in the course of treatment of the patient, it may be necessary to repeat the same laboratory test on the same day to obtain subsequent (multiple) test results. under these circumstances, the laboratory test performed can be identified by its usual procedure number and the addition of modifier 91. note: this modifier may not be used when tests are rerun to confirm initial results; due to testing problems with specimens or equipment; or for any other reason when a normal, one-time, reportable result is all that is required. this modifier may not be used when other code(s) describe a series of test results (eg, glucose tolerance tests, evocative/suppression testing). this modifier may only be used for laboratory test(s) performed more than once on the same day on the same patient.
XU Unusual non-overlapping service, the use of a service that is distinct because it does not overlap usual components of the main service
26 Professional component: certain procedures are a combination of a physician or other qualified health care professional component and a technical component. when the physician or other qualified health care professional component is reported separately, the service may be identified by adding modifier 26 to the usual procedure number.
79 Unrelated procedure or service by the same physician or other qualified health care professional during the postoperative period: the individual may need to indicate that the performance of a procedure or service during the postoperative period was unrelated to the original procedure. this circumstance may be reported by using modifier 79. (for repeat procedures on the same day, see modifier 76.)
AY Item or service furnished to an esrd patient that is not for the treatment of esrd
CR Catastrophe/disaster related
GV Attending physician not employed or paid under arrangement by the patient's hospice provider
GX Notice of liability issued, voluntary under payer policy
Q0 Investigational clinical service provided in a clinical research study that is in an approved clinical research study
Q4 Service for ordering/referring physician qualifies as a service exemption
QQ Ordering professional consulted a qualified clinical decision support mechanism for this service and the related data was provided to the furnishing professional
XS Separate structure, a service that is distinct because it was performed on a separate organ/structure
Date
Action
Notes
2011-01-01 Changed Short description changed.
2001-01-01 Changed Code description changed.
Pre-1990 Added Code added.
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