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Official Description

Creatine

© Copyright 2026 American Medical Association. All rights reserved.

Common Language Description

The CPT® Code 82540 refers to a laboratory test that measures the levels of creatine in the blood or urine. Creatine is a nitrogenous organic acid that plays a crucial role in energy production and metabolism within the body's cells, particularly in muscle tissue. It is synthesized from amino acids and is vital for the proper functioning of muscle cells, as it helps in the regeneration of adenosine triphosphate (ATP), the primary energy carrier in cells. This test may be ordered by healthcare providers to assess for inherited metabolic disorders that can affect the body's ability to produce or utilize creatine effectively. The measurement of creatine levels can provide important insights into a patient's metabolic health. To perform this test, a blood sample is typically obtained through a procedure known as venipuncture, which is separately reportable, or a urine sample may be collected either randomly or through a timed collection method. The analysis of the samples is conducted using advanced techniques such as liquid chromatography coupled with tandem mass spectrometry, ensuring accurate and reliable results.

© Copyright 2026 Coding Ahead. All rights reserved.

1. Indications

The creatine test (CPT® Code 82540) may be ordered for several specific indications, including:

  • Evaluation of Metabolic Disorders The test is performed to assess for inherited disorders of metabolism that may affect the body's ability to produce or utilize creatine effectively.

2. Procedure

The procedure for measuring creatine levels involves several key steps, which are outlined as follows:

  • Step 1: Sample Collection A blood sample is obtained through a process known as venipuncture, which involves inserting a needle into a vein to draw blood. Alternatively, a urine sample may be collected, which can be done either randomly or through a timed collection method, depending on the specific requirements of the test.
  • Step 2: Sample Preparation Once the blood or urine samples are collected, they are prepared for analysis. This may involve processing the blood to separate serum or plasma, which are the components that will be tested for creatine levels.
  • Step 3: Testing Methodology The prepared samples are then analyzed using liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS). This advanced testing technique allows for precise measurement of creatine levels in the samples, ensuring accurate results.

3. Post-Procedure

After the creatine test is performed, there are generally no specific post-procedure care requirements for patients. However, it is important for healthcare providers to review the test results in conjunction with the patient's clinical history and other diagnostic findings. Patients may be advised to follow up with their healthcare provider to discuss the results and any further necessary evaluations or treatments based on the findings of the test.

Short Descr ASSAY OF CREATINE
Medium Descr ASSAY OF CREATINE
Long Descr Creatine
Status Code Statutory Exclusion (from MPFS, may be paid under other methodologies)
Global Days XXX - Global Concept Does Not Apply
PC/TC Indicator (26, TC) 9 - Not Applicable
Multiple Procedures (51) 9 - Concept does not apply.
Bilateral Surgery (50) 9 - Concept does not apply.
Physician Supervisions 09 - Concept does not apply.
Assistant Surgeon (80, 82) 9 - Concept does not apply.
Co-Surgeons (62) 9 - Concept does not apply.
Team Surgery (66) 9 - Concept does not apply.
Diagnostic Imaging Family 99 - Concept Does Not Apply
CLIA Waived (QW) No
APC Status Indicator Conditionally packaged laboratory tests
Type of Service (TOS) 5 - Diagnostic Laboratory
Berenson-Eggers TOS (BETOS) T1H - Lab tests - other (non-Medicare fee schedule)
MUE 1
CCS Clinical Classification 233 - Laboratory - Chemistry and Hematology
90 Reference (outside) laboratory: when laboratory procedures are performed by a party other than the treating or reporting physician or other qualified health care professional, the procedure may be identified by adding modifier 90 to the usual procedure number.
GW Service not related to the hospice patient's terminal condition
GA Waiver of liability statement issued as required by payer policy, individual case
59 Distinct procedural service: under certain circumstances, it may be necessary to indicate that a procedure or service was distinct or independent from other non-e/m services performed on the same day. modifier 59 is used to identify procedures/services, other than e/m services, that are not normally reported together, but are appropriate under the circumstances. documentation must support a different session, different procedure or surgery, different site or organ system, separate incision/excision, separate lesion, or separate injury (or area of injury in extensive injuries) not ordinarily encountered or performed on the same day by the same individual. however, when another already established modifier is appropriate it should be used rather than modifier 59. only if no more descriptive modifier is available, and the use of modifier 59 best explains the circumstances, should modifier 59 be used. note: modifier 59 should not be appended to an e/m service. to report a separate and distinct e/m service with a non-e/m service performed on the same date, see modifier 25.
91 Repeat clinical diagnostic laboratory test: in the course of treatment of the patient, it may be necessary to repeat the same laboratory test on the same day to obtain subsequent (multiple) test results. under these circumstances, the laboratory test performed can be identified by its usual procedure number and the addition of modifier 91. note: this modifier may not be used when tests are rerun to confirm initial results; due to testing problems with specimens or equipment; or for any other reason when a normal, one-time, reportable result is all that is required. this modifier may not be used when other code(s) describe a series of test results (eg, glucose tolerance tests, evocative/suppression testing). this modifier may only be used for laboratory test(s) performed more than once on the same day on the same patient.
GZ Item or service expected to be denied as not reasonable and necessary
Q1 Routine clinical service provided in a clinical research study that is in an approved clinical research study
Q6 Service furnished under a fee-for-time compensation arrangement by a substitute physician or by a substitute physical therapist furnishing outpatient physical therapy services in a health professional shortage area, a medically underserved area, or a rural area
Date
Action
Notes
2013-01-01 Changed Medium Descriptor changed.
Pre-1990 Added Code added.
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Description
Code
Description
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