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Official Description

b-Hexosaminidase, each assay

© Copyright 2026 American Medical Association. All rights reserved.

Common Language Description

The CPT® Code 83080 refers to the laboratory test for measuring b-Hexosaminidase levels in serum and leukocytes. This test is crucial for assessing the activity of the b-Hexosaminidase enzyme, which plays a significant role in the hydrolysis of hexosamine. Hexosaminidase exists in three functional lysosomal forms: Hex A, Hex B, and Hex S. Hex A is particularly important as its deficiency is associated with Tay-Sachs disease and its variants, while a total deficiency of both Hex A and Hex B is indicative of Sandhoff disease and its variants. Hex S, on the other hand, is a minor component and does not contribute to the pathology of Sandhoff disease. The test involves obtaining a blood sample through venipuncture, which is a separately reportable procedure. The serum is analyzed using heat inactivation combined with fluorometric and automated techniques, while leukocytes are tested using heat inactivation with fluorometric and semiautomated methods. Each assay performed for b-Hexosaminidase levels should be reported using the code 83080.

© Copyright 2026 Coding Ahead. All rights reserved.

1. Indications

The b-Hexosaminidase assay is performed for specific clinical indications related to lysosomal storage disorders. The following conditions may warrant this test:

  • Tay-Sachs Disease - A genetic disorder caused by a deficiency of Hex A, leading to the accumulation of GM2 gangliosides in the brain and nervous system.
  • Sandhoff Disease - A more severe form of GM2 gangliosidosis characterized by a deficiency of both Hex A and Hex B, resulting in similar but more pronounced neurological symptoms.
  • Variants of Tay-Sachs and Sandhoff Diseases - Other genetic mutations that may affect Hex A and Hex B levels, necessitating testing for accurate diagnosis and management.

2. Procedure

The procedure for conducting the b-Hexosaminidase assay involves several key steps to ensure accurate measurement of enzyme levels:

  • Step 1: Blood Sample Collection - A blood sample is obtained from the patient through a venipuncture, which is a standard procedure for drawing blood. This step is crucial as it provides the necessary serum and leukocyte samples for testing.
  • Step 2: Serum Testing - The serum portion of the blood sample is subjected to heat inactivation to eliminate any interfering substances. Following this, fluorometric and automated techniques are employed to measure the b-Hexosaminidase levels accurately.
  • Step 3: Leukocyte Testing - The leukocyte fraction is also heat inactivated to prepare it for testing. Similar to the serum analysis, fluorometric and semiautomated methods are utilized to determine the enzyme activity in the leukocytes.

3. Post-Procedure

After the b-Hexosaminidase assay is completed, the results are analyzed and interpreted by a qualified healthcare professional. Depending on the findings, further diagnostic testing or genetic counseling may be recommended, especially if a lysosomal storage disorder is suspected. Patients may be advised on follow-up appointments to discuss the results and any necessary treatment options. It is important to ensure that the results are documented accurately for future reference and potential clinical decision-making.

Short Descr ASSAY OF B HEXOSAMINIDASE EA
Medium Descr ASSAY OF B-HEXOSAMINIDASE EACH ASSAY
Long Descr b-Hexosaminidase, each assay
Status Code Statutory Exclusion (from MPFS, may be paid under other methodologies)
Global Days XXX - Global Concept Does Not Apply
PC/TC Indicator (26, TC) 9 - Not Applicable
Multiple Procedures (51) 9 - Concept does not apply.
Bilateral Surgery (50) 9 - Concept does not apply.
Physician Supervisions 09 - Concept does not apply.
Assistant Surgeon (80, 82) 9 - Concept does not apply.
Co-Surgeons (62) 9 - Concept does not apply.
Team Surgery (66) 9 - Concept does not apply.
Diagnostic Imaging Family 99 - Concept Does Not Apply
CLIA Waived (QW) No
APC Status Indicator Conditionally packaged laboratory tests
Type of Service (TOS) 5 - Diagnostic Laboratory
Berenson-Eggers TOS (BETOS) T1H - Lab tests - other (non-Medicare fee schedule)
MUE 2
CCS Clinical Classification 233 - Laboratory - Chemistry and Hematology
90 Reference (outside) laboratory: when laboratory procedures are performed by a party other than the treating or reporting physician or other qualified health care professional, the procedure may be identified by adding modifier 90 to the usual procedure number.
Date
Action
Notes
2023-01-01 Note Short description changed.
2013-01-01 Changed Medium Descriptor changed.
1999-01-01 Added First appearance in code book in 1999.
Code
Description
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