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Official Description

Phosphogluconate, 6-, dehydrogenase, RBC

© Copyright 2026 American Medical Association. All rights reserved.

Common Language Description

The CPT® Code 84085 refers to the measurement of 6-phosphogluconate dehydrogenase (6PGD) levels in a blood sample. This enzyme plays a critical role in the pentose phosphate pathway, which is essential for cellular metabolism, particularly in red blood cells (RBCs). The process involves the conversion of 6-phosphogluconate into ribulose 5-phosphate, a key step in generating NADPH and ribose-5-phosphate, which are vital for various biosynthetic processes and maintaining cellular health. A deficiency in 6PGD can result in significant metabolic disturbances, particularly under stress conditions, leading to episodic hemolytic anemia. This condition is characterized by the destruction of red blood cells, which can occur during periods of increased metabolic demand or oxidative stress. Therefore, measuring the levels of 6PGD is crucial for diagnosing potential enzyme deficiencies and understanding their implications in RBC metabolism and overall health.

© Copyright 2026 Coding Ahead. All rights reserved.

1. Indications

The measurement of 6-phosphogluconate dehydrogenase (6PGD) levels is indicated in the following scenarios:

  • Genetic Deficiency Diagnosis The test is performed to diagnose a genetic deficiency of the 6PGD enzyme, which can lead to metabolic disorders.
  • Evaluation of Hemolytic Anemia It is indicated for patients presenting with symptoms of hemolytic anemia, particularly when there is a suspicion of enzyme deficiencies contributing to red blood cell destruction.
  • Assessment During Stress Events The test may be indicated during episodes of stress or illness, where increased hemolysis is suspected due to metabolic demands on red blood cells.

2. Procedure

The procedure for measuring 6-phosphogluconate dehydrogenase (6PGD) levels involves several key steps:

  • Step 1: Blood Sample Collection A blood sample is collected from the patient, typically via venipuncture. This sample is essential for the subsequent analysis of 6PGD levels.
  • Step 2: Laboratory Processing The collected blood sample is processed in a laboratory setting. This may involve separating the red blood cells from the plasma to isolate the components necessary for the enzyme assay.
  • Step 3: Enzyme Activity Measurement The laboratory performs a specific assay to measure the activity of 6-phosphogluconate dehydrogenase in the red blood cells. This assay quantifies the enzyme's activity, which is indicative of its levels in the sample.
  • Step 4: Result Interpretation Once the assay is complete, the results are interpreted by a qualified healthcare professional. The enzyme activity levels are compared against established reference ranges to determine if a deficiency is present.

3. Post-Procedure

After the procedure, the patient may be advised to resume normal activities unless otherwise directed by their healthcare provider. The results of the 6PGD test will typically be reviewed in conjunction with the patient's clinical history and other laboratory findings to assess for any potential enzyme deficiencies. If a deficiency is identified, further evaluation and management may be necessary to address the underlying condition and prevent complications associated with hemolytic anemia.

Short Descr ASSAY OF RBC PG6D ENZYME
Medium Descr PHOSPHOGLUCONATE 6-DEHYD RBC
Long Descr Phosphogluconate, 6-, dehydrogenase, RBC
Status Code Statutory Exclusion (from MPFS, may be paid under other methodologies)
Global Days XXX - Global Concept Does Not Apply
PC/TC Indicator (26, TC) 9 - Not Applicable
Multiple Procedures (51) 9 - Concept does not apply.
Bilateral Surgery (50) 9 - Concept does not apply.
Physician Supervisions 09 - Concept does not apply.
Assistant Surgeon (80, 82) 9 - Concept does not apply.
Co-Surgeons (62) 9 - Concept does not apply.
Team Surgery (66) 9 - Concept does not apply.
Diagnostic Imaging Family 99 - Concept Does Not Apply
CLIA Waived (QW) No
APC Status Indicator Conditionally packaged laboratory tests
Type of Service (TOS) 5 - Diagnostic Laboratory
Berenson-Eggers TOS (BETOS) T1H - Lab tests - other (non-Medicare fee schedule)
MUE 1
CCS Clinical Classification 233 - Laboratory - Chemistry and Hematology
Date
Action
Notes
Pre-1990 Added Code added.
Code
Description
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