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Official Description

Riboflavin (Vitamin B-2)

© Copyright 2026 American Medical Association. All rights reserved.

Common Language Description

The CPT® Code 84252 refers to a laboratory test that measures the levels of riboflavin, also known as Vitamin B2, in the blood. Riboflavin is an essential micronutrient that plays a critical role in various bodily functions, including growth and the production of red blood cells (RBCs). It is vital for the metabolism of macronutrients such as fats, proteins, and carbohydrates, as well as ketone bodies, which are produced during fat metabolism. Adequate daily intake of riboflavin is necessary to maintain optimal health, and deficiencies in this vitamin can lead to health issues, including anemia. The test involves obtaining a blood sample through a procedure known as venipuncture, which is reported separately. The plasma obtained from the blood sample is then analyzed using a technique called quantitative high-performance liquid chromatography (HPLC), which allows for precise measurement of riboflavin levels in the plasma. This test is important for diagnosing riboflavin deficiency and monitoring the nutritional status of patients.

© Copyright 2026 Coding Ahead. All rights reserved.

1. Indications

The riboflavin (Vitamin B2) blood test, coded as CPT® 84252, is indicated for the assessment of riboflavin levels in patients who may be experiencing symptoms related to deficiency or for monitoring nutritional status. The following conditions may warrant this test:

  • Riboflavin Deficiency Symptoms such as sore throat, redness and swelling of the lining of the mouth and throat, cracks or sores on the outsides of the lips (cheilosis) and at the corners of the mouth (angular stomatitis), inflammation and redness of the tongue (magenta tongue), and a lack of energy.
  • Anemia Evaluation in patients presenting with anemia, as decreased riboflavin levels can contribute to the development of this condition.
  • Malnutrition Assessment in individuals with malnutrition or those at risk of nutritional deficiencies due to dietary restrictions or absorption issues.

2. Procedure

The procedure for obtaining a riboflavin level involves several key steps that ensure accurate measurement of the vitamin in the blood. The following procedural steps are outlined:

  • Step 1: Patient Preparation Prior to the blood draw, the patient may be instructed to avoid certain foods or supplements that could interfere with the test results. It is essential to ensure that the patient is adequately hydrated and comfortable during the procedure.
  • Step 2: Venipuncture A qualified healthcare professional performs venipuncture to collect a blood sample. This involves using a sterile needle to puncture a vein, typically in the arm, to draw blood into a collection tube. The site is cleaned with an antiseptic to minimize the risk of infection.
  • Step 3: Sample Handling After the blood is collected, the sample is properly labeled and handled according to laboratory protocols. It is crucial to ensure that the sample is processed promptly to maintain the integrity of the riboflavin measurement.
  • Step 4: Laboratory Analysis The plasma obtained from the blood sample is subjected to quantitative high-performance liquid chromatography (HPLC). This analytical technique separates and quantifies riboflavin levels in the plasma, providing precise results that can be interpreted by healthcare providers.

3. Post-Procedure

After the blood sample has been collected, the patient may experience minor discomfort or bruising at the venipuncture site, which typically resolves quickly. There are no specific post-procedure care instructions required for this test, but patients should be advised to report any unusual symptoms, such as excessive bleeding or signs of infection at the puncture site. The results of the riboflavin level test will be communicated to the healthcare provider, who will interpret the findings in the context of the patient's overall health and nutritional status.

Short Descr ASSAY OF VITAMIN B-2
Medium Descr ASSAY OF RIBOFLAVIN-VITAMIN B-2
Long Descr Riboflavin (Vitamin B-2)
Status Code Statutory Exclusion (from MPFS, may be paid under other methodologies)
Global Days XXX - Global Concept Does Not Apply
PC/TC Indicator (26, TC) 9 - Not Applicable
Multiple Procedures (51) 9 - Concept does not apply.
Bilateral Surgery (50) 9 - Concept does not apply.
Physician Supervisions 09 - Concept does not apply.
Assistant Surgeon (80, 82) 9 - Concept does not apply.
Co-Surgeons (62) 9 - Concept does not apply.
Team Surgery (66) 9 - Concept does not apply.
Diagnostic Imaging Family 99 - Concept Does Not Apply
CLIA Waived (QW) No
APC Status Indicator Conditionally packaged laboratory tests
Type of Service (TOS) 5 - Diagnostic Laboratory
Berenson-Eggers TOS (BETOS) T1H - Lab tests - other (non-Medicare fee schedule)
MUE 1
CCS Clinical Classification 233 - Laboratory - Chemistry and Hematology
90 Reference (outside) laboratory: when laboratory procedures are performed by a party other than the treating or reporting physician or other qualified health care professional, the procedure may be identified by adding modifier 90 to the usual procedure number.
GA Waiver of liability statement issued as required by payer policy, individual case
26 Professional component: certain procedures are a combination of a physician or other qualified health care professional component and a technical component. when the physician or other qualified health care professional component is reported separately, the service may be identified by adding modifier 26 to the usual procedure number.
59 Distinct procedural service: under certain circumstances, it may be necessary to indicate that a procedure or service was distinct or independent from other non-e/m services performed on the same day. modifier 59 is used to identify procedures/services, other than e/m services, that are not normally reported together, but are appropriate under the circumstances. documentation must support a different session, different procedure or surgery, different site or organ system, separate incision/excision, separate lesion, or separate injury (or area of injury in extensive injuries) not ordinarily encountered or performed on the same day by the same individual. however, when another already established modifier is appropriate it should be used rather than modifier 59. only if no more descriptive modifier is available, and the use of modifier 59 best explains the circumstances, should modifier 59 be used. note: modifier 59 should not be appended to an e/m service. to report a separate and distinct e/m service with a non-e/m service performed on the same date, see modifier 25.
77 Repeat procedure by another physician or other qualified health care professional: it may be necessary to indicate that a basic procedure or service was repeated by another physician or other qualified health care professional subsequent to the original procedure or service. this circumstance may be reported by adding modifier 77 to the repeated procedure or service. note: this modifier should not be appended to an e/m service.
91 Repeat clinical diagnostic laboratory test: in the course of treatment of the patient, it may be necessary to repeat the same laboratory test on the same day to obtain subsequent (multiple) test results. under these circumstances, the laboratory test performed can be identified by its usual procedure number and the addition of modifier 91. note: this modifier may not be used when tests are rerun to confirm initial results; due to testing problems with specimens or equipment; or for any other reason when a normal, one-time, reportable result is all that is required. this modifier may not be used when other code(s) describe a series of test results (eg, glucose tolerance tests, evocative/suppression testing). this modifier may only be used for laboratory test(s) performed more than once on the same day on the same patient.
GW Service not related to the hospice patient's terminal condition
GY Item or service statutorily excluded, does not meet the definition of any medicare benefit or, for non-medicare insurers, is not a contract benefit
GZ Item or service expected to be denied as not reasonable and necessary
Q4 Service for ordering/referring physician qualifies as a service exemption
XU Unusual non-overlapping service, the use of a service that is distinct because it does not overlap usual components of the main service
Date
Action
Notes
2021-01-01 Note Guidelines changed.
2013-01-01 Changed Medium Descriptor changed.
2011-01-01 Changed Guideline information changed.
Pre-1990 Added Code added.
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