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Official Description

17-hydroxypregnenolone

© Copyright 2026 American Medical Association. All rights reserved.

Common Language Description

The CPT® Code 84143 refers to the laboratory test for measuring levels of 17-hydroxypregnenolone, a steroid hormone that plays a crucial role in the synthesis of other hormones, including cortisol and sex steroids. This complex molecule is classified as a C-21 steroid and is produced primarily in the adrenal glands and gonads through a biochemical process known as hydroxylation of pregnenolone. The measurement of 17-hydroxypregnenolone is significant as its levels peak at the end of puberty and subsequently decline with age. Notably, during pregnancy, the levels of this hormone increase, reflecting its involvement in various physiological processes. The test is particularly valuable in clinical settings for diagnosing congenital adrenal hyperplasia, a genetic disorder affecting adrenal hormone production. Additionally, it is utilized to evaluate women presenting with symptoms such as hirsutism, which is excessive hair growth, and infertility, providing essential information for further medical assessment and management. The test is conducted by obtaining a blood sample through venipuncture, which is a separately reportable procedure. The serum or plasma obtained from the blood sample is then analyzed using advanced techniques such as quantitative high-performance liquid chromatography-tandem mass spectrometry, ensuring accurate and reliable measurement of hormone levels.

© Copyright 2026 Coding Ahead. All rights reserved.

1. Indications

The 17-hydroxypregnenolone test is indicated for several clinical scenarios, particularly in the evaluation of hormonal imbalances and related conditions. The following are the primary indications for performing this test:

  • Congenital Adrenal Hyperplasia This test aids in the diagnosis of congenital adrenal hyperplasia, a genetic disorder characterized by enzyme deficiencies that lead to abnormal steroid hormone production.
  • Hirsutism The test is utilized to evaluate women who exhibit signs of hirsutism, which is the presence of excessive hair growth in areas where men typically grow hair, indicating potential hormonal imbalances.
  • Infertility It is also indicated for women experiencing infertility, as abnormal levels of 17-hydroxypregnenolone may contribute to reproductive issues.

2. Procedure

The procedure for obtaining a 17-hydroxypregnenolone test involves several key steps to ensure accurate results. The following outlines the procedural steps:

  • Step 1: Patient Preparation Prior to the blood draw, the patient may be instructed to follow specific guidelines, such as fasting or avoiding certain medications, to ensure the accuracy of the test results.
  • Step 2: Venipuncture A qualified healthcare professional performs a venipuncture, which involves inserting a needle into a vein, typically in the arm, to collect a blood sample. This step is crucial as the quality of the sample directly impacts the test results.
  • Step 3: Sample Handling After the blood is drawn, the sample is properly labeled and handled according to laboratory protocols to prevent contamination or degradation of the specimen.
  • Step 4: Laboratory Analysis The collected serum or plasma is then subjected to quantitative high-performance liquid chromatography-tandem mass spectrometry, a sophisticated analytical technique that allows for precise measurement of 17-hydroxypregnenolone levels in the sample.

3. Post-Procedure

After the blood sample has been collected, there are generally no specific post-procedure care requirements for the patient. However, patients may be advised to apply pressure to the venipuncture site to minimize bruising and to keep the area clean and dry. Results from the test are typically available within a specified timeframe, and healthcare providers will discuss the findings with the patient to determine any necessary follow-up actions or treatments based on the hormone levels measured.

Short Descr ASSAY OF 17-HYDROXYPREGNENO
Medium Descr 17-HYDROXYPREGNENOLONE
Long Descr 17-hydroxypregnenolone
Status Code Statutory Exclusion (from MPFS, may be paid under other methodologies)
Global Days XXX - Global Concept Does Not Apply
PC/TC Indicator (26, TC) 9 - Not Applicable
Multiple Procedures (51) 9 - Concept does not apply.
Bilateral Surgery (50) 9 - Concept does not apply.
Physician Supervisions 09 - Concept does not apply.
Assistant Surgeon (80, 82) 9 - Concept does not apply.
Co-Surgeons (62) 9 - Concept does not apply.
Team Surgery (66) 9 - Concept does not apply.
Diagnostic Imaging Family 99 - Concept Does Not Apply
CLIA Waived (QW) No
APC Status Indicator Conditionally packaged laboratory tests
Type of Service (TOS) 5 - Diagnostic Laboratory
Berenson-Eggers TOS (BETOS) T1H - Lab tests - other (non-Medicare fee schedule)
MUE 2
CCS Clinical Classification 233 - Laboratory - Chemistry and Hematology
90 Reference (outside) laboratory: when laboratory procedures are performed by a party other than the treating or reporting physician or other qualified health care professional, the procedure may be identified by adding modifier 90 to the usual procedure number.
Q4 Service for ordering/referring physician qualifies as a service exemption
Date
Action
Notes
1994-01-01 Added First appearance in code book in 1994.
Code
Description
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